The effect of pretreatment on the invertase activity of gel-entrapped yeast biomass |
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Authors: | Liza Pira Carmina Pinto Carlos Rolz |
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Affiliation: | (1) Facultad de Ingenieria, Universidad Rafael Landivar, Guatemala;(2) Center for Scientific and Technological Studies, Central American Research Institute for Industry (ICAITI), P.O. Box 1552, 01901 Guatemala, Guatemala |
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Abstract: | Summary Gel-entrapped, non-viable yeast biomass with specific invertase activity has been produced by two different pretreatment protocols: a short-time thermal treatment and a brief contact with concentrated ethanol solutions. Four yeast strains were most promising:K. fragilis L-293,C. utilis L-282,S. cerevisiae L-170 and L-209. Of these, the ethanol-tolerant L-282 and the ethanol-tolerant and heat-resistant L-170 gave the most active gel-entrapped biocatalysts: around 2 mg of reducing sugars produced per mg dry yeast per min. |
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Keywords: | Invertase Entrapped yeast Ethanol pretreatment Heat pretreatment |
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