Involvement in DNA repair of the ruvA gene of Escherichia coli |
| |
Authors: | Hiroshi Iwasaki Toshikazu Shiba Atsuo Nakata and Hideo Shinagawa |
| |
Institution: | (1) Department of Experimental Chemotherapy, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, 565 Suita, Osaka, Japan |
| |
Abstract: | Summary The ruv operon of Escherichia coli consists of two genes, orfl1 and ruv, which encode 22 and 37 kilodalton proteins, respectively, and are regulated by the SOS system. Although the distal gene, ruv, is known to be involved in DNA repair, the function of orf1 has not been studied. To examine whether orf1 is also involved in DNA repair, we constructed a strain with a deletion of the entire ruv operon. The strain was sensitive to UV even after introduction of low copy number plasmids carrying either orf1 or ruv, but UV resistance was restored by introduction of a plasmid carrying both orfl and ruv. These results suggest that orf1 as well as ruv is involved in DNA repair. Therefore, orf1 and ruv should be renamed ruvA and ruvB, respectively. |
| |
Keywords: | Complementation test ruv operon Recombination DNA repair SOS function |
本文献已被 SpringerLink 等数据库收录! |
|