Expression analysis of plant intracellular Ras-group related leucine-rich repeat proteins (PIRLs) in Arabidopsis thaliana |
| |
Authors: | Md. Firose Hossain Mst Momtaz Sultana Ai Tanaka Amit Kumar Dutta Takushi Hachiya Tsuyoshi Nakagawa |
| |
Affiliation: | 1. Department of Molecular and Functional Genomics, Interdisciplinary Center for Science Research, Shimane University, Matsue, 690-8504, Japan;2. Bioresource and Life Sciences, The United Graduate School of Agricultural Sciences, Tottori University, Tottori, 680-8550, Japan;3. Department of Agricultural Extension (DAE), Ministry of Agriculture, Dhaka, 1215, Bangladesh;4. Department of Microbiology, University of Rajshahi, Rajshahi, 6205, Bangladesh |
| |
Abstract: | Arabidopsis thaliana contains a family of nine genes known as plant intracellular Ras-group related leucine-rich repeat (LRR) proteins (PIRLs). These are structurally similar to animals and fungal LRR proteins and play important roles in developmental pathways. However, to date, no detailed tissue-specific expression analysis of these PIRLs has been performed. Therefore, in this study, we generated promoter:GUS transgenic plants for the nine A. thaliana PIRL genes and identified their expression patterns in seedlings and floral organs at different developmental stages. Most PIRL members showed expression in the root apical region and in the vascular tissue of primary and lateral roots. Shoot apex-specific expression was recorded for PIRL1 and PIRL8. Furthermore, PIRL1, PIRL3, PIRL5, PIRL6, and PIRL7 showed distinct expression patterns in flowers, especially in pollen and anthers. In addition, co-expression network analysis identified cases where PIRLs were co-expressed with other genes known to have specific functions related to growth and development. Taken together, the tissue-specific expression patterns of PIRL genes improve our understanding of the functions of this gene family in plant growth and development. |
| |
Keywords: | Leucine-rich repeat Promoter:GUS assay Co-expression Phylogenetic analysis Pollen |
本文献已被 ScienceDirect 等数据库收录! |
|