Nitrergic Modulation of Hippocampal Acetylcholine Release Is Independent of Endothelial NO Synthase Activity |
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Authors: | Buchholzer Marie-Luise Klein Jochen |
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Institution: | (1) Department of Pharmacology, Johannes Gutenberg University of Mainz, Mainz, Germany;(2) Department of Pharmaceutical Sciences, Texas Tech School of Pharmacy, Amarillo, Texas, 79106 |
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Abstract: | Nitric oxide (NO) is involved in hippocampal phenomena of synaptic plasticity. The present microdialysis study investigated a possible role of NO and of endothelial NO synthase (eNOS) activity in the control of hippocampal acetylcholine (ACh) release. 3-Morpholinosydnonimine (SIN-1), an NO donor, stimulated ACh release by 50%–70% when infused into the hippocampus of wild type C57B16 mice. Infusion of l-nitroarginine (L-NA), a broad-spectrum inhibitor of NO synthases, decreased hippocampal ACh efflux by approximately 50%. Mice lacking eNOS (eNOS knockouts) had identical basal effluxes of hippocampal ACh as wild type mice, and the responses to SIN-1 and L-NA were unchanged in the absence of eNOS activity. We conclude that nitric oxide (NO) stimulates hippocampal ACh release in a tonic fashion, but independently of eNOS activity. |
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Keywords: | Mice microdialysis nitric oxide nitroarginine SIN-1 |
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