Expression and thermostability of Paenibacillus campinasensis BL11 pectate lyase and its applications in bast fibre processing |
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| Authors: | C.‐H. Ko C.‐H. Tsai J. Tu S.‐H. Tang C.‐C. Liu |
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| Affiliation: | 1. School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan;2. Bioenergy Research Center, National Taiwan University, Taipei, Taiwan;3. Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan;4. Strong Biotech Corp., Taipei, Taiwan |
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| Abstract: | An open reading frame (ORF) with 963 nucleotides from Paenibacillus campinasensis BL11 was cloned and expressed in Escherichia coli. It encodes a pectate lyase (EC 4.2.2.2) of 35.6 kDa, denominated Pel‐BL11. The recombinant Pel‐BL11 was fused with His‐tag and purified. An optimal activity of 1623 IU mg?1 was exhibited at 50°C, pH 10. Significant activities of Pel‐BL11 are demonstrated between 40 and 70°C and from a pH of 7–11. The observed half‐lives are 103 min at 70°C and 288 min at 40°C. Compared to other published acid and alkaline pectate lyases, Pel‐BL11 demonstrated exceptional thermostability and wider pH adaptability. Temperature effects on the cleavage of the pectate α‐1,4‐glycosidic bond by Pel‐BL11 were examined. Continuous cleavage occurred for the first 3 h at 30 and 50°C. However, at 70°C, the majority of the cleavage occurred during the first 10 min. Weight loss in gampi and paper mulberry fibres after enzyme treatment validate the potential of this treatment in fibre degumming. |
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| Keywords: | Bast fibre Paenibacillus campinasensis pectate lyase pectin retting |
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