Three-dimensional structure of the Chinese Sacbrood bee virus |
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Authors: | ZHANG Jingqiang FENG Jianxun LIANG Yuyao ZHANG Qinfen Lu Xinying Chen Donghua Z.H.Zhou |
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Affiliation: | 1. State Key Laboratory for Bio-control, Zhongshan University, Guangzhou 510275, China 2. Department of Pathology and Laboratory Medicine, University of Texas-Houston Medicine School, Houston, TX 77030, USA |
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Abstract: | The RNA of Chinese Sacbrood Bee Virus (CSBV) was purified and used as template to obtain a 1096 bp cDNA fragment by RT-PCR amplification. This DNA fragment was cloned into pGEM-T Easy Vector for sequencing. Analyses of the sequenced CSBV RNA fragment revealed a nucleotide sequence homology of 87.6% and a deduced amino-acid sequence homology of 94.6% with that of the Sacbrood Virus (SBV), indicating that CSBV is a different but highly homologous virus of SBV. The three-dimensional (3D) structure of CSBV was determined at 2.5 nm resolution by using electron cryo-microscopy (cryoEM) and computer reconstruction methods. The 3-D structure showed that the capsid has aT = 1 (orP = 3) icosahedral capsid shell with a smooth surface. There were 12 pentons at its icosahedral vertices (5-fold axes) and 132 holes penetrating the shell. The 3-D structure also revealed densities corresponding to the CSBV genome, suggesting icosahedrally-ordered RNA organization, a novel feature not previously reported for any picornaviruses. |
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