Changes of peroxidase,esterase isozyme activities and some cell inclusions in regenerated vascular tissues after girdling inBroussonetia papyrifera (L.) Vent

The isozyme zymogram of peroxidase and esterase, and some cell inclusion contents were changed with the differentiation of regenerated vascular stem tissues after girdling inBroussnetia papyrifera (L.). Vent. Presence or disappearance of some peroxidase and esterase isozyme bands was related to wounding. Some isoperoxidase bands disappeared at the time of vascular tissue formation, but some esterase isozyme bands appeared in phloem or cambial regions as sieve-like elements or mature xylem were formed. The inclusion grains progressively disappeared with the formation of callus and initiation of vascular meristems. The cell inclusions reappeared during the formation of regenrated vascular tissues. Histochemical study indicated that the inclusion grains could be a complex compound of a protein mass encircling polysaccharide in the center with a proteinous nucleus.