Agrobacterium-mediated transformation of centipedegrass (Eremochloa ophiuroides [Munro] Hack.) |
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| Authors: | Mingxi?Liu,Shaoyun?Lu,Lin?Liu,Jiali?Tan,Zhenfei?Guo author-information" > author-information__contact u-icon-before" > mailto:zhfguo@scau.edu.cn" title=" zhfguo@scau.edu.cn" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
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| Affiliation: | (1) State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China;(2) Biotechnology Laboratory for Turfgrass and Forages, College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China; |
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| Abstract: | Centipedegrass (Eremochloa ophiuroides [Munro] Hack.) is an important warm-season turfgrass and pasture grass. Due to availability of an efficient regeneration system, this study was undertaken to develop an Agrobacterium-mediated transformation system for centipedegrass for potential use in its genetic improvements. Embryogenic calli were co-cultivated with Agrobacterium strain EHA105 harboring pCAMBIA1301, followed by selection of hygromycin B (hyg B)-resistant callus and regenerated plantlets. The transformants were analyzed using PCR, DNA blot, RNA blot and semi-quantitative RT-PCR. Two transformant lines, lines 2 and 6, showed integration and expression of the hyg B resistance gene hpt. Line 2 revealed a single integration of the hpt transgene, while line 6 revealed two integration sites of hpt. Both lines exhibited resistance to hyg B, although line 2 showed higher level of expression of hpt. |
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