Biosynthesis in vitro and the pattern of lectin binding receptors in monkey kidney cell surfaces. |
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Authors: | M Basu J R Moskal D A Gardener S Basu |
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Institution: | 1. Department of Chemistry, Biochemistry and Biophysics Program, University of Notre Dame, Notre Dame, Indiana 46556 USA;2. Research Division, Miles Laboratories, Inc., Elkhart, Indiana 46514 USA |
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Abstract: | The glycosyltransferase activities involved in the biosynthesis of neutral blood group-related glycosphingolipids were measured in African green monkey kidney cells (Vero) grown in culture. The a-fucosyltransferases which catalyzed the reaction between GDP-fucose and corresponding acceptors to form H-active and novel Lea-type glycosphingolipids were characterized in membrane fractions isolated from Vero cells and monkey bone marrow. Using 125I-labeled and lectins the differential binding to Vero cell surface glycoproteins and glycolipids was studied under various conditions. |
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