Synthesis of tetracontapeptide--a hypothetical evolutionary precursor of calcium-binding proteins. II. Condensation of fragments |
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Authors: | R Jung V N Medvedkin Iu V Mitin |
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Abstract: | A tetracontapeptide was obtained by condensation of synthetic fragments (see the preceding paper) with the use of pentafluorophenyl esters as well as with carbodiimide and hydroxybenzotriazole. Racemization during fragment condensation was 1-2 per cent. Deblocking of the protected tetracontapeptide was carried out by treatment with trifluoroacetic acid and hydrogen fluoride with thioanisole. The obtained peptide was purified by gel-chromatography and HPLC. |
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