Comparison of umbilical vein models for measurement of relative prostacyclin and thromboxane production |
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Authors: | Paulette Mehta Jawahar Mehta Fulton Crews Louis Roy Nancy Ostrowski Christine Horalek |
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Institution: | Departments of Pediatrics, Medicine, and Pharmacology University of Florida College of Medicine Gainesville, Florida 32610, USA |
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Abstract: | There is growing evidence that blood vessels generate TXA2 in addition to PGI2. We examined effluents from continously perfused human umbilical vein and supernatants from umbilical vein rings for TXB2 and 6-keto-PGF1α measurements (stable metabolites of TXA2 and PGI2, respectively). TXB2 and 6-keto-PGF1α were identified in all samples. 6-keto-PGF1α to TXB2 ratio was higher in intact vein effluents than in the venous ring supernatants (112:1 and 28:1, respectively, P<0.01). Arachidonate stimulation increased 6-keto-PGF1α and TXB2 levels similarly in the intact vein effluent. In contrast, stimulation of the venous rings resulted in a relatively larger increase in TXB2 than in 6-keto-PGF1α. This caused 6-keto-PGF1α to TXB2 ratio to decline (p<0.01). The identity of TXB2 was confirmed in several different ways. These data suggest that 1) human umbilical veins produce TXA2 in addition to PGI2, 2) TXA2 release is more by venous rings than by the intact vein probably reflecting contribution from non-endothelial layers, and 3) arachidonate stimulation causes relatively greater release of TXA2 than of PGI2 from the venous rings, whereas release of PGI2 and TXA2 is similar from the intact vein. |
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