Roles of cortactin, an actin polymerization mediator, in cell endocytosis |
| |
Authors: | Chen Li Wang Zhi-Wei Zhu Jian-wei Zhan Xi |
| |
Institution: | Department of Pathology, School of Medicine, Nantong University, Nantong 226001, China. usazhujianwei@yahoo.com.cn. |
| |
Abstract: | Cortactin, an actin-binding protein and a substrate of Src, is encoded by the EMS1 oncogene. Cortactin is known to activate Arp2/3 complex-mediated actin polymerization and interact with dynamin, a large GTPase and proline rich domain-containing protein. Transferrin endocytosis was significantly reduced in cells by knock-down of cortactin expression as well as in vivo introduction of cortactin immunoreagents. Cortactin-dynamin interaction displayed morphologically dynamic co-distribution with a change in the endocytosis level in cells treated with an actin depolymerization reagent, cytochalasin D. In an in vitro beads assay, a branched actin network was recruited onto dynamin-coated beads in a cortactin Src homology domain 3 (SH3)-dependent manner. In addition, cortactin was found to function in the late stage of clathrin coated vesicle formation. Taken together, cortactin is required for optimal clathrin mediated endocytosis in a dynamin directed manner. |
| |
Keywords: | cortactin dynamin actin polymerization endocytosis |
本文献已被 维普 PubMed 等数据库收录! |
|