The kinetics of fructose utilization byS. cerevisiae IGC 4261 in sucrose adjunct brewers wort fermentations

Summary Fructose utilization in laboratory-scale sucrose adjunct brewers wort fermentations, using the brewing strainS. cerevisiae IGC 4261, is predicted by a mathematical model based on the kinetic parameters of the membrane transport proteins which affect fructose uptake into the cell. These include biphasic fructose transport via a proton symport and the constitutive hexose facilitated diffusion system, plus the competitive inhibitory effect that glucose has on this latter component. Also the non-competitive inhibitory effects of a) maltose on fructose uptake via its proton symport and b) ethanol on biphasic fructose transport are incorporated within the model, as well as the inoculum size.