Odorant-sensitive phospholipase C in insect antennae |
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Authors: | I Boekhoff J Strotmann K Raming E Tareilus H Breer |
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Institution: | Institute of Zoophysiology, University Stuttgart-Hohenheim, F.R.G. |
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Abstract: | Exogenous tritiated phosphatidylinositol bisphosphate added to antennal preparations from locust and cockroach was hydrolysed releasing inositol trisphosphate. High activity of phospholipase C was detected in the soluble as well as in the membrane fraction. At low free calcium concentrations hydrolysis of the labelled lipid was stimulated by odorants and pheromones in a GTP-dependent manner. Consequently the level of inositol trisphosphate in antennal preparations increased upon odorant stimulation. |
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