加工番茄种质资源的SSR分析

为探明加工番茄种质资源间的亲缘关系,利用SSR标记对20份加工番茄品种及育种材料进行了多样性分析。结果表明:从49对SSR引物中筛选出12对扩增稳定、条带清晰且多态性丰富的引物进行分析,共获得43个位点,多态性位点为37个,多态位点比率86%;供试材料间的遗传相似系数介于0.419~1.000之间,说明加工番茄种质资源间存在一定的遗传差异;通过UPGMA法聚类分析,将20份材料分为3大类群,其中亲缘关系较远的不同类群间及亚类间的种质资源可作为杂交育种的亲本。

Processing Tomato Germplasm Analyzed by SSR Markers

Simple Sequence Repeats (SSR) marker was used to analyze genetic diversity of 20 samples of processing tomato for evaluation of genetic relationship. The results show that a total of 43 bands were scored from the 12 primers, which were screened out from 49 primers and demonstrated highly reproducible, clear and polymorphic bands. Among the obtained bands, 37 were polymorphic, accounting for 86% of the total. Analyzed by NTSYS2.2, the genetic similarity coefficient among the accessions ranged from 0.419 to 1.000, which indicated that genetic basis was relatively abundance among the 20 processing tomato resources. A dendrogram was constructed by UPGMA cluster method. The 20 samples of processing tomato were classified into 3 major groups, the materials that have a distant genetic relationship between major groups or sub-major groups, which may be used as elite materials for further genetic improvement in this species.