Biotransformation of chlorpyrifos and bioremediation of contaminated soil |
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Authors: | C Vidya Lakshmi Mohit Kumar Sunel Khanna |
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Institution: | aDepartment of Biotechnology and Bioinformatics, NIIT Institute of Information Technology (TNI), Balaji Estate, Kalkaji, New Delhi 110019, India;bDepartment of Biotechnology and Environmental Sciences, Thapar Institute of Engineering and Technology, Patiala 147004, Punjab, India |
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Abstract: | Five aerobic consortia capable of degrading chlorpyrifos as a sole carbon source in aqueous medium showed degradation in the range of 46–72% after 20 days. Pseudomonas fluorescence, Brucella melitensis, Bacillus subtilis, Bacillus cereus, Klebsiella species, Serratia marcescens and Pseudomonas aeroginosa, isolated from these consortium, showed 75–87% degradation of chlorpyrifos as compared to 18% in control after 20 days of incubation. Bioremediation of chlorpyrifos-contaminated soil with P. fluorescence, B. melitensis, B. subtilis and P. aeroginosa individually showed 89%, 87%, 85% and 92% degradation, respectively, as compared to 34% in control after 30 days. Population dynamics of the introduced isolates based on antibiotic resistance survival and REP-PCR indicated 60–70% survival based on antibiotic resistance, but only 35–45% of the inoculated population based on REP-PCR. During bioremediation studies, 3,5,6-trichloro-2-pyridinol (TCP) was detected as metabolite of chlorpyrifos degradation by P. aeroginosa after 20 days, which was utilized and disappeared after 30 days. Whole-cell studies also showed that P. aeroginosa gave TCP as the product of chlorpyrifos degradation, which was further metabolized to unknown polar metabolites.Scientific relevancePotential application in sites for effective in situ bioremediation of chlorpyrifos, a neurotoxic insecticide widely used in India. |
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Keywords: | Chlorpyrifos degradation Bioremediation Microcosm REP-PCR Population survival TCP degradation |
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