Somatic embryogenesis in cultured mature zygotic embryos of Abies balsamea |
| |
Authors: | Todd G Guevin Victor Micah Edward G Kirby |
| |
Institution: | (1) Department of Biological Sciences, Rutgers University, University Heights, 07102 Newark, New Jersey, USA |
| |
Abstract: | Embryo suspensor masses (ESMs) were induced by culture of isolated mature zygotic embryos of balsam fir Abies balsamea (Mill.)] on media containing 10 M cytokinin 6-(--dimethylallylamino)purine (2iP), 6-benzyladenine (BA), or thidiazuron (TDZ)]. Once induced, ESMs proliferated on media containing 2iP, BA or TDZ (10 M) or on 4.5 M BA in combination with 10 M naphthyl-1-acetic acid. When ESMs were transferred to media containing 5–80 M abscisic acid, cotyledonary-stage embryos were formed. Embryos were readily germinated on medium lacking growth regulators.Abbreviations ABA
abscisic acid
- BA
6-benzyladenine
- ESM
embryo-suspensor mass
- 2iP
6-(--dimethylallylamino)purine
- NAA
naphthyl-1-acetic acid
- TDZ
N-phenyl-N-1,2,3-thiadiazol-5-yl urea (thidiazuron) |
| |
Keywords: | conifers cytokinins |
本文献已被 SpringerLink 等数据库收录! |
|