Comparison of combinations of diluents and media for enumerating Zygosaccharomyces rouxii in intermediate water activity foods |
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| Authors: | UM Abdul-Raouf CA Hwang LR Beuchat |
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| Institution: | Center for Food Safety and Quality Enhancement, Department of Food Science and Technology, University of Georgia, Griffin, GA, USA |
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| Abstract: | Combinations of five diluents (0.1% peptone, 40 and 50% glucose, and 18 and 26% glycerol) and three enumeration media (tryptone glucose yeast extract, dichloran 18% glycerol and malt extract yeast extract 50% glucose (MY50G) agars) were evaluated for recovering a xerotolerant yeast, Zygosaccharomyces rouxii , from foods with intermediate water activity ( a w). Combinations of 40% ( a w, 0.936) or 50% ( a w 0.898) glucose diluent and MY50G agar ( a w 0.890) were superior in recovering highest populations. The type of solute in the diluent, as well as a reduced a w, influences efficiency of recovering viable cells. |
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