In vitro regeneration and micro-propagation of enset from Southwestern Ethiopia |
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Authors: | Negash Almaz Puite Klaas Schaart Jan Visser Bert Krens Frans |
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Institution: | (1) Plant Research International, P.O. Box 16, Wageningen, the, Netherlands;(2) Institute for Biodiversity Conservation and Research, P.O. Box, 30726 Addis Ababa, Ethiopia |
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Abstract: | Three clones of enset (Ensete ventricosum Welw. Cheesman) from southwestern Ethiopia (Keffa-Shaka zone) were investigated for their potential for micropropagation
and regeneration in tissue culture. Corm and leaf tissue of greenhouse-grown plants as well as in vitro germinated zygotic embryos were used as starting material for micro-propagation and regeneration studies. Embryos were excised
from disinfected seeds and cultured in vitro. Multiple shoots from both corm- and embryo-explants were obtained using regeneration medium supplemented with 10 μM or 20
μM BAP. Rooting of shoots was achieved using medium with 5 μM IBA, 1 μM BAP and 1 g l−1 activated charcoal. Plantlets obtained by this process were transferred to soil under greenhouse conditions. Optimal conditions,
which were determined for clonal propagation of three different genotypes of enset, allowing both in vitro micropropagation and regeneration, are described. This protocol makes for conservation of enset clones, rapid propagation
of selected disease-free germplasm and more efficient breeding procedures.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | corm embryo enset Ensete ventricosum Welw Cheesman germination leaf explants micropropagation regeneration |
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