A novel microbial interaction: obligate commensalism between a new gram-negative thermophile and a thermophilic Bacillus strain |
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Authors: | Sung-Keun Rhee Seung-Goo Lee Seung-Pyo Hong Yoon-Ho Choi Jong-Hoon Park Chul-Joong Kim M-H Sung |
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Institution: | (1) Microbial Conversion RU., Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Korea Tel. +82-42-860-4372; Fax +82-42-860-4595 e-mail: smoonhee@mail.kribb.re.kr, KR;(2) Dept. of Veterinary Medicine, Chungnam National University, 220 Kung-Dong, Taejon, Korea, KR |
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Abstract: | Obligately commensal interaction between a new gram-negative thermophile and a thermophilic Bacillus strain was investigated. From compost samples, a mixed culture showing tyrosine phenol-lyase activity was enriched at 60°C.
The mixed culture consisted of a thermophilic gram-negative strain, SC-1, and a gram-positive spore-forming strain, SK-1.
In mixed cultures, strain SC-1 started to grow only when strain SK-1 entered the stationary phase. Although strain SC-1 showed
tyrosine phenol lyase activity, we could not isolate a colony with any nutrient medium. For the isolation and cultivation
of strain SC-1, we added culture supernatant and cell extract of the mixed culture to the basal medium. The supernatant and
cell extract of the mixed culture contained heat-stable and heat-labile factors, respectively, that are essential to the growth
of strain SC-1. During pure cultures of strain SK-1, the heat-stable growth factors were released during the growth phase
and the heat-labile growth factors were produced intracellularly at the early stationary phase. Strain SC-1 was gram-negative
and microaerophilic, and grows optimally at 60°C. Based on these results, we propose a novel commensal interaction between
a new gram-negative thermophile, strain SC-1, and Bacillus sp. strain SK-1.
Received: November 18, 1999 / Accepted: December 2, 1999 |
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Keywords: | Gram-negative thermophile Tyrosine phenol-lyase Commensalism Growth factors |
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