Histochemistry of Tobacco Mesophyll Cell Walls Pretreated with Bacterial Protein-lipopolysaccharides

Prevention of hypersensitive confluent-necrosis in tobacco mesophyll, caused by Pseudomonas syringae pv. aptata, and tolerance towards the incompatible bacterium are induced 48 h after intercellular injection of protein-lipopolysaccharide complexes. Histochemical analysis and ultrastructural observations were carried out to determine whether there was an association between this tolerance state and cell wall alterations due to callose, lignin and/or suberin deposition either before or after challenge with the incompatible bacterium. No evidence was obtained for such wall alterations.