Biosynthesis of penicillin V acylase by Fusarium sp.: effect of culture conditions |
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Authors: | V. K. Sudhakaran J. G. Shewale |
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Affiliation: | (1) Research and Development, Hindustan Antibiotics Ltd., 411 018 Pimpri, Pune, India |
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Abstract: | Penicillin V acylase was produced, both intracellularly and extracellularly, by Fusarium sp. SKF 235 grown in submerged fermentation. When neopeptone was added to the medium, >95% of the penicillin V acylase was extracellular. In the absence of a complex organic nitrogen source, the fungus produced low levels of totally intracellular penicillin V acylase. MgSO4 was essential for synthesis of the enzyme, which was induced by phenoxyacetic acid and penicillin V. The maximum yield of penicillin V acylase was 430 IU/g dry cell wt. The optimum pH value and temperature for the penicillin V acylase were 6.5 and 55°C, respectively. |
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Keywords: | 6-Aminopenicillanic acid Fusarium sp. penicillin acylase penicillin V acylase |
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