Isolation and structure of d-xylans from pericarp seeds of Opuntia ficus-indica prickly pear fruits |
| |
Authors: | Youssef Habibi Mostafa MahrouzMichel R. Vignon |
| |
Affiliation: | a Centre de Recherches sur les Macromolécules Végétales, CNRS, and Université Joseph Fourrier, BP 53, F-38041 Grenoble cedex 9, France b Faculté des Sciences Semlalia (Université Cadi Ayyad), BP 2390, Marrakech, Morocco |
| |
Abstract: | Xylans were isolated from the pericarp of prickly pear seeds of Opuntia ficus-indica (OFI) by alkaline extraction, fractionated by precipitation and purified. Six fractions were obtained and characterized by sugar analysis and NMR spectroscopy. They were assumed to be (4-O-methyl-d-glucurono)-d-xylans, with 4-O-α-d-glucopyranosyluronic acid groups linked at C-2 of a (1→4)-β-d-xylan. The sugar composition and the 1H and 13C NMR spectra showed that their chemical structures were very similar, but with different proportions of d-Xyl and 4-O-Me-d-GlcA. Our results showed that, on average, the water soluble xylans have one nonreducing terminal residue of 4-O-methyl-d-glucuronic acid for every 11 to 14 xylose units, whereas in the water non-soluble xylans, xylose units can varied from 18 to 65 residues for one nonreducing terminal residue of 4-O-methyl-d-glucuronic acid. |
| |
Keywords: | Cactus Opuntia ficus-indica Prickly pear seeds 4-O-Methyl-glucuronoxylan 1H and 13C NMR spectroscopy |
本文献已被 ScienceDirect 等数据库收录! |
|