Oligomerization reactions of deoxyribonucleotides on montmorillonite clay: The effect of mononucleotide structure on phosphodiester bond formation |
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Authors: | James P Ferris Kamaluddin |
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Institution: | (1) Department of Chemistry, Rensselaer Polytechnic Institute, 12180-3590 Troy, NY, USA |
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Abstract: | Adenine deoxynucleotides bind more strongly to Na+-montmorillonite than do the corresponding ribonucleotides. Thymidine nucleotides binds less strongly to Na+-montmorillonite than do the corresponding adenine deoxynucleotides. Oligomers of 2 -dpA up to the tetramer were detected in the reaction 2 -d-5 -AMP with EDAC (a water-soluble carbodiimide) in the presence of Na+-montmorillonite. Reaction of 3 -d-5 -AMP with EDAC on Na+-montmorillonite yields 3 -d-2 ,5 -pApA while the reaction of 2 -d-3 -AMP yields almost exclusively 3 ,5 -cdAMP. The reaction of 5 -TMP under the same reaction conditions give 3 ,5 -cpTpT and 3 ,5 -pTpT while 3 -TMP gives mainly 3 ,5 -cpT. The yield of dinucleotide products (dpNpN) containing the phosphodiester bond is 1% or less when Na+-montmorillonite is omitted from the reaction mixture. |
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