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A new monoclonal antibody (3D3) generated with human respiratory mucins and directed against Lewis determinants |
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| Authors: | Nathalie Emery; Palfa Shirley B; Place Graham; Oriol Rafael; Hall Roderick L; Roussel Philippe; Lhermitte Michel |
| Institution: | Unit INSERM no. 377, place de Verdun, 59045 Lille Cedex, France 1Bayer Ltd, Pharma Research Stoke Poges, Slough, UK 2 Unit INSERM no. 178, avenue Paul-Vaillant-Couturier, Villejuif, France |
| Abstract: | We have prepared a monoclonal antibody (MAb), 3D3, raised againstpurified human respiratory mucins. This antibody recognizedmucins and proteolytically derived glycopeptides. The epitoperecognized by the antibody was destroyed by  -L-fucosidase, indicatingthat it was present on the carbohydrate moieties. Structuralspecificity was determined by adsorption on a variety of synthetic,insolubilized oligosaccharides. Several lines of evidence indicatethat the 3D3 MAb reacted strongly with the Lewis (Leb) antigen,but also recognized Lea and Ley determinants. This antibodymight be useful to study mucin secretion. human bronchial mucins Lewis b |
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