Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of microencapsulated osteoblasts under hypoxic condition |
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| Authors: | Kedong Song Guofeng Zhao Tianqing Liu Lei Zhang Xuehu Ma Jing Liu Zhanfeng Cui |
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| Institution: | (1) Dalian R&D Center for Stem Cell and Tissue Engineering, State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China;(2) Department of Neuromedicine, First Affiliated Hospital, Dalian Medical University, Dalian, 116021, China;(3) Department of Engineering Science, Oxford University, Parks Road, Oxford, OX1 3PJ, UK |
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| Abstract: | The expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB) with the support of microencapsulated
osteoblasts under hypoxia environment was investigated. The expansion of HSPCs was evaluated through the total number of UCB
mononuclear cells (MNCs) produced, their repopulating potential with the colony-forming unit assay (CFU-Cs) and CD34+ phenotypic analysis with flow cytometry. At the end of 7 days of culture, the UCB-MNCs, CFU-Cs and CD34+ cells had achieved 18.7 ± 1.6, 11.6 ± 0.9 and 23.4 ± 2-fold expansions, respectively, in the test groups. These were significantly
different from those in control groups. Microencapsulated osteoblasts under hypoxia conditions had therefore a significant
effect on the expansion potential of HSPCs in vitro. |
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| Keywords: | Hematopoietic stem cells Hypoxia Microcapsulation Osteoblasts Stem cells |
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