Immunoelectron microscopic localization of one of the spore germination proteins, GerAB, in Bacillus subtilis spores. |
| |
Authors: | Y Sakae Y Yasuda and K Tochikubo |
| |
Abstract: | Ultrastructural localization of GerAB, one of the proteins of Bacillus subtilis spores related to L-alanine-initiated germination, was investigated by immunoelectron microscopy with antipeptide (residues 61 to 80 of GerAB) antiserum and a colloidal gold-immunoglobulin G complex. Immunogold particles were visualized in the boundary region between the cortex and coat of dormant spores, and they were broadly dispersed into the cortex region after germination. |
| |
Keywords: | |
|
|