Haloperidol reduces K+-evoked Ca2+-dependentd-[3H]aspartate release from rat hippocampal slices |
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Authors: | E. Tzavara R. Svarna G. Palaiologos |
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Affiliation: | (1) Laboratory of Biological Chemistry, Medical School University of Athens, Mikras Asias 75, GR11527 Athens, Greece |
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Abstract: | Rat hippocampal slices preloaded withd-[3H]aspartate, a non metabolizable analogue ofl-glutamate, were superfused with artifical CSF. Depolarization was induced by 53.5 mM K+, in the presence of Ca2+ (1.3 mM) or Mg2+ (5 mM) to determine the Ca2+ dependent release. Haloperidol added in the superfusion medium at 100 M reduced by about 60% the Ca2+ dependent release ofd-[3H]aspartate. This drug at 20 M or 100 M inhibited the non-activated glutamate dehydrogenase (GDH) but had no effect on GDH activated by ADP (2 mM) or leucine (5 mM). In addition no effect was observed on phosphate activated glutaminase (PAG) in the presence either of 20 mM or 5 mM phosphate. These results indicate that the effect of haloperidol is exerted on presynaptic mechanisms regulating neurotransmitter release. |
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Keywords: | Haloperidol font-variant:small-caps" >d-aspartate hippocampus Ca2+ dependent glutamate release glutamate dehydrogenase phosphate activated glutaminase |
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