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Thymidine phosphorylase activity of anucleate minicells of Escherichia coli immobilized in an agarose gel matrix |
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| Authors: | G G Khachatourians J D Brosseau J J Child |
| Institution: | (1) Biotechnology Laboratory, Department of Applied Microbiology and Food Science, University of Saskatchewan, S7N OW0 Saskatoon, Canada;(2) Present address: Chemistry & Biology Division, Saskatchewan Research Council, Campus Drive, S7N 0W0 Saskatoon, Canada;(3) Present address: Operational Planning, National Research Council, Montreal Road, K1A OR6 Ottawa, Canada |
| Abstract: | Escherichia coli whole cells, and minicells were immobilized on columns in an agarose gel matrix and evaluated for their thymidine phosphorylase (TPP) activity. The greatest specific activity of TPP was found with the induced minicells (2.2  moles thymine/min/mg protein) at a column retention time of 11 min. |
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