A structured model for the kinetics of fungal amylase production |
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Authors: | D E Brown S W Fitzpatrick |
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Institution: | (1) Department of Chemical Engineering, U.M.I.S.T., Manchester, England |
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Abstract: | Summary The cell is considered to be divided into nucleic acids, proteinaceous material and storage compounds. The enzyme is believed to be constitutive but repressed by the rate of catabolism. A structured model is developed to describe the growth, amylase production and dissolved oxygen profile in the batch culture ofAspergillus oryzae.Nomenclature CA
Conc. of enzyme SKB units/m3
- CD,CE,CG,CO,CS
Conc. of D.E.G. mass, oxygen, substrate kg/m3
- CO*
Mean oxygen conc. in gas-liquid interface kg/m3
- CX
Total cell conc. kg/m3
- D
D-mass (proteins) kg
- E
E-mass (storage carbon) kg
- G
G-mass (necleic acids) kg
- KE
Rate of usage of E-mass kg/m3/h
- KEO
KE with oxygen limitation kg/m3/h
- Q
Active fraction of promotor genes -
- RD,RE,RG
Rate of production of D,E,G-mass kg/m3/h
- RO,RS
Rate of usage of oxygen, substrate kg/m3/h
- RDO,REO,RGO
RD,RE and RG with oxygen limitation kg/m3/h
- S
Substrate (carbon) concentration kg/m3
- t
Time h
- to
Time at which CS = 0 h
- K1,K2,K3,K24,K25
Stoichiometric constants -
- K4,K5,K6,K7
Rate constants h–1
- K8-K12,K18,K20-K23
Michaelis-Menten constants kg/m3
- K26,K26
Absorption coefficient, K26 at CX = 0 h–1
- K27
Empirical constant kg/m3
- K15
Rate of enzyme formation SKB units/kg
- K16,K17
Equilibrium constants m3/kg
- K19
Decay constant for mRNA h–1 |
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Keywords: | |
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