Changing antigenic pattern of tubulin-containing structures during spreading ofMizuhopecten yessoensis (Mollusca) hemocytes |
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Authors: | E Korenbaum I Linhartová V Viklický |
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Institution: | (1) Institute of Marine Biology, Far East Science Center, Academy of Sciences of the USSR, Vladivostok;(2) Insitute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague |
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Abstract: | Summary The immunoreactivity of a panel of anti-tubulin monoclonal antibodies with spreadingMizuhopecten yessoensis hemocytes was studied by immunofluorescence and immunoblotting. In immunoblotting all the antibodies used reacted only with bands corresponding to the position of tubulin subunits. Hemocytes showed a reorganization of microtubules and microfilaments during cell spreading. In spread-out cells the TU-04 antibody stained microtubules growing out of the centriole in the cell body; in contrast to TU-07 and TU-10 antibodies, which stained microspike-like bundles on the periphery of the cells. The presence of microfilaments in microspikes was detected by rhodamine-labeled phalloidin.Abbreviations CB
cytoskeletal buffer
- SWAM-FITC
fluorescein isothiocyanate-labeled swine anti mouse immunoglobulin
- MTOC
microtubule organizing centers
- SDS-PAGE SDS
polyacrylamide gel electrophoresis |
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Keywords: | Cytoskeleton Monoclonal antibodies Cell spreading Immunoblotting Immunofluorescence |
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