Rapid detection of human papilloma virus using a novel leaky surface acoustic wave peptide nucleic acid biosensor |
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Authors: | Yunxia Wang Ming Chen Liqun Zhang Yi Ding Yang Luo Qinghua Xu Jianfeng Shi Liang Cao Weiling Fu |
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Affiliation: | aDepartment of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, PR China;bDepartment of Laboratory Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, PR China;cThe 26th Research Institute, Chinese Electronic Scientific and Technical Group Company, Chongqing 400060, PR China |
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Abstract: | A novel leaky surface acoustic wave (LSAW) bis-peptide nucleic acid (bis-PNA) biosensor with double two-port resonators has been constructed successfully for the quantitative detection of human papilloma virus (HPV). The bis-PNA probe can directly detect HPV genomic DNA without polymerase chain reaction (PCR) amplification, and it can bind to the target DNA sequences more effectively and specifically than a DNA probe. When the concentrations varied from 1 pg/L to 1000 μg/L, with 100 μg/L being the optimal, a typical linearity was found between the quantity of target and the phase shifts. The detection limit was 1.21 pg/L and the clinical specificity was 97.22% of that of real-time PCR. The bis-PNA probe was able to distinguish sequences that differ only in one base. Both the intraassay and interassay coefficients of variance (CVs) were <10%, and the biosensor can be regenerated for ten times without appreciable loss of activity. Therefore, this technical platform of LSAW biosensor can be applied to clinical samples for direct HPV detection. |
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Keywords: | Leaky surface acoustic wave (LSAW) Peptide nucleic acid (PNA) Biosensor Human papilloma virus (HPV) |
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