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Rice ubiquitin promoters: deletion analysis and potential usefulness in plant transformation systems |
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| Authors: | J?Wang |
| Institution: | (1) Department of Agronomy, AgCenter Biotechnology Laboratory, Louisiana Agricultural Experiment Station, LSU Agricultural Center, Louisiana State University, Baton Rouge, LA, 70803, USA;(2) Present address: Genetics Programm and Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474, USA |
| Abstract: | Strong constitutive promoters form a cornerstone for basic and applied research using transgenic plants. GUS (beta-glucuronidase) expression levels from constructs containing RUBQ1 or RUB2 rice ubiquitin promoters were 8- to 35-fold higher in transgenic rice Oryza sativa (L.)] plants, respectively, when compared to the 35S promoter. Deletion analysis of the 5'-upstream region of RUBQ2 revealed a putative enhancer region that produced a 2.4-fold increase in transient GUS expression. Southern blot analysis showed that three to seven copies of the GUS gene were stably inserted into R0 and R1 plants and inherited in a monogenic fashion. |
| Keywords: | Gene silencing Promoter Oryza sativa Transgenic plant Ubiquitin |
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