Voltage-clamp measurement of visually-evoked conductances with whole-cell patch recordings in primary visual cortex |
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| Institution: | 1. Department of Ophthalmology, The First Affiliated Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi 710061, China;2. Institute of Neurobiology, Xi’an Jiaotong University Health Science Center, Xi’an, Shaanxi 710061, China;3. Ningbo Medical Treatment Center Lihuili Hospital, Ningbo, Zhejiang 315040, China;1. Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, PA, USA;2. Department of Neuroscience and Center for Neurovirology, Temple University School of Medicine, Philadelphia, PA, USA;3. Comprehensive NeuroAIDS Center, Temple University School of Medicine, Philadelphia, PA, USA;1. Animal Model Research Center, Jeonbuk Department of Inhalation Research, Korea Institute of Toxicology, Jeongup, Republic of Korea;2. School of Mechanical Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea;3. Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea;4. Departement of Neurosurgery, Presbyterian Medical Center, Jeonju, Republic of Korea;1. Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, 13th Street, Charlestown, MA 02129, USA;2. Surgical Oncology, Department of Medicine, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA;3. Department of Veterans Affairs, VA Boston Healthcare System, Boston, MA 02130, USA;4. Department of Neurology, Boston University School of Medicine, Boston, MA 02118, USA |
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| Abstract: | Whole cell patch recordings have been realized in the primary visual cortex of the anesthetized and paralyzed cat, in order to better characterize input resistance and time constant of visual cortical cells in vivo. Measurements of conductance changes evoked by visual stimulation were derived from voltage clamp recordings achieved in continuous mode at two or more different subtreshold holding potentials. They show that the magnitude of the conductance increase can reach up to 300% of the mean conductance at rest. The observation of similar changes for the preferred and antagonist responses, when flashing ON and OFF, a test stimulus in pure ON and OFF subfields supports the hypothesis of a role for shunting inhibition in the spatial organization of simple receptive fields. |
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