Manganese peroxidase production in submerged cultures by free and immobilized mycelia of Nematoloma frowardii |
| |
| Authors: | Rogalski J Szczodrak J Janusz G |
| |
| Affiliation: | 1. Department of Biochemistry, Maria Curie-Skłodowska University, 3 M.C.-Skłodowska Square, 20-031 Lublin, Poland;2. Department of Industrial Microbiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland;1. Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 675-8501, Japan;2. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan;3. Biomass Engineering Program, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan;4. Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea;1. Faculty of Chemical Science and Engineering, China University of Petroleum, Beijing, PR China;2. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, PR China;1. lmmanuel Kant Baltic Federal University, 3 Botkin str., 236016 Kaliningrad, Russia;2. Institute of Clinical Immunology, 14 Ydrintsevskaya str., 630099 Novosibirsk, Russia;1. School of Environmental Science & Engineering, Tianjin University, Tianjin 300350, China;2. College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China;3. Organic Recycling Institute (Suzhou) of China Agricultural University, Wuzhong District, Suzhou 215128, China;4. Beijing Advanced Innovation Center for Big Data-based Precision Medicine, Beihang University, Beijing 100191, China;5. Institute of Agricultural Sciences in Taihu Lake District, Suzhou Academy of Agricultural Sciences, Suzhou 215155, China;1. College of Resources and Environment, Northeast Agricultural University, Harbin, 150030, China;2. College of Agriculture, Haeju Kim JeWon University of Agriculture, Haeju, 999093, Democratic People''s Republic of Korea;3. Department of Chemistry and Biochemistry and the UCLA-Department of Energy Institute for Genomics and Proteomics, UCLA, Los Angeles, California 90095;4. Howard Hughes Medical Institute;5. Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas 66502 |
| |
| Abstract: | The agaric basidiomycete Nematoloma frowardii has been suggested as a good alternative for production of the extracellular ligninolytic enzyme, manganese-dependent peroxidase (MnP). Some cultural and environmental factors influencing the enzymatic activity in shaken flasks and aerated fermenter cultures were evaluated to improve the yields of the process. A low nitrogen medium (1.36 mM N added as ammonium tartrate), containing 16 g/l glucose (C/N ratio=65.3), 2mM Mn2+ and inoculated with immobilized polyurethane foam mycelium, made it possible to obtain a MnP yield of 2304 nkat/l in 8 days. Under these operational conditions, the enzyme productivity in the immobilized cells of N. frowardii was 1.4 times higher than that obtained with the free fungus. In the procedure with the reusable immobilized mycelium (semi-continuous culture) as many as three subsequent 10 day batches could be fermented by using the same carrier with no loss of MnP activity. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
