Effects of Selective Alkylphosphorylation of Propionylcholinesterase on the Regeneration of Acetylcholinesterase in the Aqueous Soluble Fraction of Superior Cervical Ganglia of the Rat Following Sarin |
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Authors: | Winifred Angenent Koelle Kathleen Kitto Rickard George B Koelle |
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Institution: | Department of Pharmacology, Medical School/G3, University of Pennsylvania, Philadelphia, Pennsylvania, 19104 |
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Abstract: | Abstract: The rates of regeneration of acetylcholinesterase (AChE) and propionylcholinesterase (PrChE) in the supernatants of aqueous homogenates of rat superior cervical ganglia, centrifuged at 100,000 g for 90 min, were determined at 1,3, 6, and 16 h following their inactivation (>90%) by administration of sarin, 2.0μmol/kg i.v. Values were compared with those in animals in which the PrChE was continually suppressed by the repeated, fractional administration of iso-OMPA, in a total dose of 10 or 20 μmol/kg i.p. These doses of iso-OMPA alone produced 96–99% inactivation of PrChE with no detectable effect on AChE. Significant suppression of AChE regeneration by iso-OMPA administration was noted only at 6 h; in contrast with earlier findings in the cat, administration of iso-OMPA alone caused no significant increase in ganglionic AChE activity. |
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Keywords: | Acetylcholinesterase Propionylcholinesterase Pseudocholinesterase Superior Cervical Ganglion |
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