Stimulation and inhibition of insulin release by an amino-reactive probe of plasma membrane |
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Authors: | Bo Hellman Lars-Åke Idahl Åke Lernmark Janove Sehlin Inge-Bert Täljedal |
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Institution: | (1) Department of Histology, University of Umeå, Umeå, Sweden |
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Abstract: | Summary 4-Acetamido-4 -isothiocyanostilbene-2,2 -disulphonic acid (SITS), an amino-reacting probe of plasma membranes, stimulated the release of insulin from micro-dissected pancreatic islets ofob/ob-mice. This effect of SITS was inhibited by adrenaline or by calcium deficiency. SITS did not inhibit the insulin-releasing action of glucose or leucine but rather potentiated the effect of glucose. In contrast, SITS markedly depressed the insulin secretory response to chloromercuribenzene-p-sulphonic acid. It is suggested that by reacting with the plasma membranes SITS may induce secretagogic ionic fluxes in the -cells. In addition, SITS apparently inhibits the secretagogic recognition of chloromercuribenzene-p-sulphonic acid, presumably by preventing the organic mercurial from reacting with certain membrane thiol groups. |
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