用识别四个GC对的限制酶研究高等担子菌线粒体DNA

本文报道了用识别四个GC对的限制性内切酶HaeⅢ、CofI、MsPI酶切高等担子菌伞菌目（Agaricales）、非褶菌目（Aphyllophorales）12个菌株的总DNA，在琼脂糖凝胶上显现线粒体DNA带谱的结果。通过对带型、累加分子量、酶切片段数和同源性的分析表明，不同种类高等担子菌的线粒体DNA变化较大．研究还显示，以多种识别四个GC对的限制酶分析高等担子菌的线粒体DNA及其它们的RFLP，比只用HaeⅢ更灵敏有效。

ANALYSIS OF HIGHER BASIDIOMYCETOUS MITOCHONDRIAL DNA WITH FOUR-GC-CUTTER RESTRICTION ENZYMES

The mitochondrial DNA band patterns emerged from basidiomycetous genomic DNA digested with four-GC-cutter restriction enzymes, HaeIII, CfoI and MspI, were reported in this paper. It was demonstrated that mtDNAs of higher basidiomycetes had abundant diversity after comparison of their band patterns, summated sizes, the number of fragments and homology among 12 strains of Agaricales, Aphyllophorales. The results also showed that the technique, displaying fungal mtDNA bands directly from their total DNA, would be more efficient and sensitive for analyses of fungal mtDNA RFLP if several four-GC-cutter restriction enzymes, instead of one, were used.