| 酿酒酵母胞内无机焦磷酸酶的分离纯化及性质 |
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| 引用本文： | 苟萍 杨寿钧. 酿酒酵母胞内无机焦磷酸酶的分离纯化及性质[J]. 微生物学报, 1998, 38(3): 229-232 |
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| 作者姓名： | 苟萍 杨寿钧 |
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| 作者单位： | 新疆大学生物系 830046；中国科学院微生物研究所 100080 |
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| 摘 要： | An inorganic pyrophosphatase (EC3.6.1.1) from Saccharomyces cerevisiae was purified to PAGE homogeneity by sonication disruption. (NH4)2SO4 fractionation and DEAE-cellulose colunm chromatography. The optimum pH and temperature of the enzyme were 7.4～7. 8 and 60℃, respectively. The Km was 19.3 mmol / L. The enzyme required Mg2+ as a cofactor for hydrolysis of pyrophosphate and was inhibited by Ca2+, Hg2+, Pb2+, Mn2+.
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| 关 键 词： | 酿酒酵母 无机焦磷酸酶 纯化与性质 |
PURIFICATION AND PROPERTIES OF INTERCELLULAR INORGANIC PYROPHOSPHTASE FROM SACCHAROMYCES CEREVISIAE |
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| Gou Ping, Yang Shoujun. PURIFICATION AND PROPERTIES OF INTERCELLULAR INORGANIC PYROPHOSPHTASE FROM SACCHAROMYCES CEREVISIAE[J]. Acta microbiologica Sinica, 1998, 38(3): 229-232 |
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| Authors: | Gou Ping Yang Shoujun |
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| Affiliation: | Institute of Mirobiology, Chinese Academy of Sciences, Beijing 100080. |
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| Abstract: | An inorganic pyrophosphatase (EC3.6.1.1) from Saccharomyces cerevisiae was purified to PAGE homogeneity by sonication disruption, (NH4)2SO4 fractionation and DEAE-cellulose column chromatography. The optimum pH and temperature of the enzyme were 7.4-7.8 and 60 degrees C, respectively. The Km was 19.3 mmol/L. The enzyme required Mg2+ as a cofactor for hydrolysis of pyrophosphate and was inhibited by Ca2+, Hg2+, Pb2+, Mn2+. |
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| Keywords: | Saccharomyces cerevisiae Inorganic pyrophosphatase Purification and properties |
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