| 脂肪酶产生菌的选育及产酶条件的优化 |
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| 引用本文： | 高修功,章克昌,曹淑桂.脂肪酶产生菌的选育及产酶条件的优化[J].微生物学报,1998,38(4):313-317. |
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| 作者姓名： | 高修功 章克昌 曹淑桂 |
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| 作者单位： | 无锡轻工大学生物工程系 214036；吉林大学酶工程国家重点实验室 130023 |
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| 摘 要： | A lipase-producing bacterium strain was isolated from soil and was identified as Pseudomonas sp.. Its lipase yield was improved 2.25-fold by combined beatment of UV irradiation and NTG. The lipase fermentation condition for the mutant strain was optimized with Plackett-Burman design and Response Surface Analysis(RSA), and the formula of the optimum medium suitable for industrial scale fermentation was thereby established. A maximum yield of 87.5 U/ ml was obtained.
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| 关 键 词： | 脂肪酶 假单胞菌 分离 诱变 优化 |
ISOLATION OF A LIPASE-PRODUCING PSEUDOMONAS STRAIN AND OPTIMIZATION OF ITS FERMENTATION CONDITIONS |
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| Gao Xiugong, Zhang Kechang, Cao Shugui.ISOLATION OF A LIPASE-PRODUCING PSEUDOMONAS STRAIN AND OPTIMIZATION OF ITS FERMENTATION CONDITIONS[J].Acta Microbiologica Sinica,1998,38(4):313-317. |
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| Authors: | Gao Xiugong Zhang Kechang Cao Shugui |
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| Institution: | Wuxi University of Light Industry, Wuxi 214036. |
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| Abstract: | A lipase-producing bacterium strain was isolated from soil and was identified as Pseudomonas sp.. Its lipase yield was improved 2.25-fold by combined beatment of UV irradiation and NTG. The lipase fermentation condition for the mutant strain was optimized with Plackett-Burman design and Response Surface Analysis(RSA), and the formula of the optimum medium suitable for industrial scale fermentation was thereby established. A maximum yield of 87.5 U/ ml was obtained. |
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| Keywords: | Lipase Pseudomonas sp Isolation Mutation Optimization |
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