| 重组人亲环素A的表达,纯化及活性测定 |
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| 引用本文： | 李芳秋 单祥年. 重组人亲环素A的表达,纯化及活性测定[J]. 微生物学报, 1998, 38(3): 193-196 |
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| 作者姓名： | 李芳秋 单祥年 |
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| 作者单位： | 南京军区南京总医院全军医学检验中心!南京，210002南京师范大学生物系(李芳秋)，南京军区南京总医院全军医学检验中心!南京，210002(赵权，武建国)，南京师范大学生物系!南京，210097(单祥年) |
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| 摘 要： | 将RT－PCR扩增得到的亲环素A（CyPA）基因片段插入原核表达载体pET11c中，得到重组质粒pET11／CyPA，转入大肠杆菌获得高效表达。Spe－PAGE分析表明，重组CyPA表达量占菌体可溶性蛋白的40％以上。经50％硫酸铵沉淀和DEAESepharoseCL－6B柱层析可纯化重组CyPA。用糜蛋白酶偶联法测定显示重组CyPA具有肽基脯氨酸顺／反异构酶活性。
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| 关 键 词： | 亲环素A 基因表达 肽基脯氨酸顺／反异构酶 |
EXPRESSION,PURIFICATION AND CHARACTERIZATION OF RECOMBINANT HUMAN CYCLOPHILIN A |
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| Li Fangqiu, Zhao Quan, Wu Jianguo. EXPRESSION,PURIFICATION AND CHARACTERIZATION OF RECOMBINANT HUMAN CYCLOPHILIN A[J]. Acta microbiologica Sinica, 1998, 38(3): 193-196 |
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| Authors: | Li Fangqiu Zhao Quan Wu Jianguo |
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| Affiliation: | Center of Medical Laboratory Sciences of PLA, Jinling Hospital, Nanjing 210002. |
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| Abstract: | Plasmid-derived expression of the human CyPA in E. colt would make it possible to obtain ample protein quantities and to avoid difficult task of obtaining human tissues for protein purification. The cDNA encoding human CyPA from MT4 lymph cell line has been cloned and an expression vector (pET11 / CyPA) has been constructed under control of the T7 promoter for efficient expression in E. coli. The recombinant CyPA is produced at 41% of total soluble cell protein, and showed the peptidyl-prodyl cis-trans isomerase activity. |
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| Keywords: | Cyclophilin A Gene expression Peptidyl-prodyl cis-trans isomerase |
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