首页 | 本学科首页   官方微博 | 高级检索  
   检索      


A new restriction endonuclease Eco31I recognizing a non-palindromic sequence
Authors:V Butkus  J Bitinait  D Ker&#x;ulyt  A Janulaitis
Institution:V. Butkus, J. Bitinaité, D. Keršulyté,A. Janulaitis
Abstract:A restriction endonuclease with a novel site-specificity has been isolated from the Escherichia coli strain RFL31. The nucleotide sequences around a single Eco31I cut on pBR322 DNA and two cuts of λ DNA have been compared. A common 5′GAGACC3′CTCTGG sequence occurs near each cleavage site. Precise mapping of the cleavages in both DNA strands places the cuts five nucleotides to the left of the upper sequence and one nucleotide to the left of the lower sequence. This enabled us to deduce the following recognition and cleavage specificity of Eco31I: 5 ′ G G T C T C N ↓ 3 ′ C C A G A G N N N N N ↑
Keywords:Restriction endonuclease  Non-symmetrical sequence  Nucleotide specificity  (E  coli)
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号