Enzyme-amplified enzyme-linked immunosorbent assay for gibberellin A4 based on the NAD-dependent redox cycle |
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| Authors: | N. Tanno M. Takanashi T. Denboh M. Abe N. Okagami |
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| Affiliation: | (1) Department of Biology, Faculty of Science, Yamagata University, 990 Yamagata, Japan;(2) Biological Institute, Faculty of Science, Tohoku University, 980 Sendai, Japan |
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| Abstract: | An antiserum against gibberellin A4 (GA4) raised in rabbits and its partially purified antibodies were used to develop radioimmunoassay (RIA) and indirect enzyme-linked immunosorbent assays (ELISAs) for GA4. Of three immunoassays tested, an ELISA based on the NAD-dependent redox cycle (enzyme-amplified ELISA) had highest sensitivity. Levels of methylated GA4 detected by this most sensitive method ranged from 0.1 fmol/assay (3.5 fg/assay) to 0.1 pmol/assay (3.5 pg/assay) suggesting applicability of this method to the detection of gibberellins in purified plant extracts. |
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| Keywords: | Bulbil Dioscorea opposita ELISA gibberellin A4 immunoassay RIA |
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