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Effects of varying tissue sizes on the efficiency of baboon ovarian tissue vitrification
Authors:Xi-Lan Lu  Jing Yu  Gang Zhang  Zeng-Tao Wei  Jun-Tao Li  Jian-Min Zhang
Affiliation:1. Department of Reproductive Medicine, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan 250000, China;2. Operating Room of Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan 250000, China;3. Department of Pain Management, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan 250000, China;4. Department of Obstetrics and Gynecology, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan 250000, China;5. Department of Reproductive Medicine, Maternal and Child Health Care Center of Shandong Province, 238 Jingshi East Road, Jinan 250000, China
Abstract:

Objective

The aim of this study was to detect the effects of varying tissue sizes on the efficiency of baboon ovarian tissue vitrification.

Study design

The percentages of morphologically normal primordial follicles and the follicles expressing bax protein in ovarian tissues after vitrification–warming were measured. Besides, the 17-β estradiol levels in the culture supernatants were measured.

Results

The percentages of morphologically normal primordial follicles in vitrified–warmed ovarian tissues slicing in 0.5–1.5 mm in length and wide, and 1.0 mm in thickness were significantly higher than those slicing in 2.0 mm in length and wide, and 1.0 mm in thickness. Moreover, the follicles expressing bax protein in vitrified–warmed ovarian tissues slicing in 0.5–1.5 mm in length and wide, and 1.0 mm in thickness were significantly lower than those slicing in 2.0 mm in length and wide, and 1.0 mm in thickness. The 17-β estradiol levels in the culture supernatants slicing in 1.0–1.5 mm in length and wide, and 1.0 mm in thickness were significantly higher than those slicing in 0.5 mm or 2.0 mm in length and wide, and 1.0 mm in thickness.

Conclusions

Cortex piece slicing in 1.0–1.5 mm in length and wide, and 1.0 mm in thickness is suitable for baboon ovarian vitrification.
Keywords:Size   Baboon   Ovarian tissue   Vitrification
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