烟草毛状根诱导及其茄尼醇含量初探

茄尼醇是合成泛醌类药物的重要中间体。以发根农杆菌(Agrobacterium rhizogenes)W.T15834感染烟草叶片诱导产生毛状根, 探讨其茄尼醇含量变化。结果显示, 获得的毛状根能在无外源生长调节剂的MS固体和液体培养基上自主生长, 但在液体培养基中培养的毛状根生长更迅速, 也不会形成愈伤组织。甘露碱检测及PCR结果证实, 发根农杆菌Ri质粒的rolB基因已在烟草(Nicotiana tabacum)毛状根基因组中整合并得到表达。用改进的HPLC法测定烟草毛状根中的茄尼醇含量, 其结果为对照根(种子萌发产生的幼苗根)的1.12倍, 但仍比废弃烟叶中茄尼醇含量低43.2％。

Preliminary Study of the Induction of Transformed Hairy Roots and Solenesol Content in Nicotiana tabacum

Solanesol is an important material for synthesizing ubiquinone. An efficient transformation system was established in Nicotiana tabacum by use of Agrobacterium rhizogenes W.T15834.The content of solanesol was detected. Hairy roots could grow normally on solid or liquid MS medium without a growth regulator. However, the hairy roots grew rapidly and no callus formed during subculture in liquid medium. The transformation of hairy roots was confirmed by PCR and mannopine assay. By use of improved HPLC, we obtained a content of solanesol of almost 43.2% in hairy roots that was 1.12 times that in roots of tobacco seedlings, but still lower than that in discarded tobacco leaves; the content of solanesol is normally higher in liquid culture than in solid culture. This study aims to provide a basis for future research in taking advantage of hairy root technology to produce solanesol.