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Histochemical demonstration of myosin Ca2+-ATPase accumulation in primary cultures of skeletal and heart muscle cells |
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| Authors: | S M Taylor and P A Jones |
| Institution: | (1) MRC Unit for Molecular and Cellular Cardiology and Department of Medical Biochemistry, University of Stellenbosch Medical School, P.O. Box 63, 7505 Tygerberg, South Africa;(2) Present address: Division of Hematology-Oncology, Childrens Hospital of Los Angeles, 4650 Sunset Boulevard, 90027 Los Angeles, California, USA |
| Abstract: | Summary A modified histochemical technique is described for the improved detection of myosin Ca2+-ATPase activity in single muscle cells in culture. The method was used to demonstrate the increase in myosin Ca2+-ATPase activity in differentiating chick skeletal muscle cells. Functional muscle cells were also positively identified in the heterogeneous cell population of primary hamster heart cell cultures. An age-dependent increase in the number of cells with high levels of myosin ATPase activity in mitotically arrested heart cell cultures was shown. Maturation of individual muscle cells could thus be evaluated. |
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