小果博落回的生物碱分离与鉴定

以D101大孔吸附树脂为吸附剂,将对小果博落回全草的酸水提取物分为50%乙醇洗脱部分和丙酮洗脱部分.对两部分洗脱物分别进行硅胶柱色谱分离,由50%乙醇洗脱部分得到5个生物碱:二氢血根碱(1),二氢白屈菜红碱(2),6-乙氧基血根碱(3),6-丙酮基血根碱(4)和Bocconarborine A (5).由丙酮洗脱部分得到原阿片碱(6),α-别隐品碱(7),β-别隐品碱(8)和小檗红碱(9).采用波谱分析对化合物进行了结构表征,结果表明,化合物3、4、5、9是首次从该植物中分离得到.

Isolation and Identification of Alkaloids from Macleaya microcarpa (Maxim.) Fedde

The method of isolation of total alkaloids from the acidic extraction solution of Macleaya microcarpa was established by using D101 macroporous resin as adsorbens to provide free alkaloid fraction A with 50% ethanol in water as eluent and free alkaloid fraction B with acetone as eluent.From the fraction A,five isoquinoline alkaloids,dihydrosanguinarine (1),dihydrochelerythrine (2),6-ethoxysanguinarine (3),6-acetonyl-dihydrosanguinarine (4) and Bocconarborine A (5) were isolated,and from the fraction B,protopine (6),α-allocryptopine (7) and β-allocryptopine (8) and berberrabine (9) was obtained,of which 3,4,5 and 9 were found in this plant for the first time.Their structures were determinated on the basis of spectroscopic evidence.