Purification and identification of endogenous anti-opioid substances from bovine brain |
| |
| Authors: | S Kaneko S Tamura H Takagi |
| |
| Affiliation: | 1. Graduate Program in Neuroscience, University of California, Riverside, CA 92521, USA;2. Department of Evolution, Ecology, and Organismal Biology, University of California, Riverside, CA 92521, USA;1. Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore, Tamil Nadu, India, 632014;2. Department of Experimental Biology, Palacky University Olomouc, Faculty of Science, Šlechtitelů 27, 78371 Olomouc, Czech Republic;3. Department of Neurology, University Hospital in Olomouc, I. P. Pavlova 6, 77520 Olomouc, Czech Republic;4. Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 5, 77515 Olomouc,Czech Republic;5. Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic;6. Laboratory of Growth Regulators, Faculty of Science, Palacký University Olomouc, and Institute of Experimental Botany of the Czech Academy of Sciences, Šlechtitelů 27, CZ-78371 Olomouc, Czech Republic;7. Department of Chemistry, Rabindranath Tagore University, Hojai, Assam, India, 782435 |
| |
| Abstract: | Two fractions with high potency for reversing the inhibitory effect of Met-enkephalin on the electrically induced contractility of guinea-pig ileum have been purified from bovine brain extract. Unexpectedly, one isolated peptide was identified as [Val5]-angiotensin I and the other fraction was [Val5]-angiotensin II, as judged by chromatographic comparisons on HPLC and amino acid analysis. Since angiotensins did not affect opioid binding to brain membrane, we consider that angiotensins may act as physiological antagonists to the opioid system in the brain, as well as in the guinea-pig ileum. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
