Growth of host dependent Bdellovibrio in host cell free system |
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Authors: | Devorah Friedberg |
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Institution: | (1) Department of Microbiological Chemistry, The Hebrew University-Hadassah Medical School, Jerusalem, Israel |
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Abstract: | A particulate, subcellular fraction of Escherichia coli was shown to promote the growth of host dependent (H-D) Bdellovibrio in the absence of host cells. The growth promoting activity was enhanced by both cations and trypsin, and destroyed by pronase. During the axenic growth unipolar spheres appear in the elongating Bdellovibrio forms. Thymidine monophosphate was more readily incorporated than thymidine into the Bdellovibrio DNA during growth in the host free system. |
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Keywords: | Bdellovibrio Axenic growth Growth factor TMP incorporation |
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