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The complete mitochondrial genome of the yeast Pichia sorbitophila
Authors:Paul P Jung  Joseph Schacherer  Jean-Luc Souciet  Serge Potier  Patrick Wincker  & Jacky de Montigny
Institution:Unitéde Génétique Moléculaire, Génomique et Microbiologie, Institut de Botanique, UMR7156, Universitéde Strasbourg, Strasbourg, France;;and Génoscope, Centre National de Séquençage, Evry, France
Abstract:Here, we report the complete nucleotide sequence of the 39 107-bp mitochondrial genome of the yeast Pichia sorbitophila . This genome is closely related to those of Candida parapsilosis and Debaryomyces hansenii , as judged from sequence similarities and synteny conservation. It encodes three subunits of cytochrome oxidase ( COX1, COX2 and COX3 ), three subunits of ATP synthase ( ATP6, ATP8 and ATP9 ), the seven subunits of NADH dehydrogenase ( NAD1-6 and NAD4L ), the apocytochrome b ( COB ), the large and small rRNAs and a complete set of tRNAs. Although the mitochondrial genome of P. sorbitophila contains the same core of mitochondrial genes observed in the ascomycetous yeasts, those coding for the RNAse P and the ribosomal protein VAR1p are missing. Moreover, the mtDNA of P. sorbitophila contains several introns in its genes and has the particularity of possessing an intron, which is not linked to any upstream exon.
Keywords:mitochondria              Pichia sorbitophila            tRNA  intron  organelle
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